MAVE resources
Multiplexed assays of variant effects (MAVEs) allow us to assess (in a single experiment) thousands of variants simultaneously. This technique, which harnesses the power of next-gen sequencing, yields large-scale data sets that can shed light on the functional consequences of protein variants and ultimately on the the landscape of human genetic variation. We are currently working on providing a comprehensive MAVE knowledge base. We hope these preliminary resources will be a helpful starting place!
If you are interested in helping to further develop this resource, please contact program manager Lara Muffley (muffley@uw.edu)
So you want to make (or use!) a variant effect map…
What tools do I need? What variables should I consider? How do I get started?
Educational Resources (clinical application)
- Introduction to Deep Mutational Scanning Animation
- Workshop Nov 2023: Functional evidence and how to use it (Villani & Spurdle)
- Workshop June 2024: Functional evidence evaluation (Villani & Spurdle)
- Interpreting Genomic Variation: Overcoming Challenges in Diverse Populations (Wellcome Connecting Science Course)
- Interested in how to use functional evidence in your clinical practice? Visit our Clinical Application page to learn how!
Educational Resources (experimental)
- Comprehensive MAVE-relevant reviews Thinking about incorporating MAVEs into your work? Visit our MAVE educational resources page to find comprehensive MAVE-relevant reviews.
- Technology and methods development manuscripts Designing a MAVE? >>Follow this link<< to view granular reviews and seminal papers relevant to methods at each stage of the MAVE development process.
Experimental Protocols
New! MAVE protocols collection in protocols.io.
https://www.protocols.io/workspaces/atlas-of-variant-effects-alliance
To add your protocol to the collection, please read this how-to guide or watch this how-to-video.
Educational Videos
- Visit (and subscribe to!) our youtube channel (which we jointly manage with CMAP/CEGS)
- Introduction to Deep Mutational Scanning Animation
- Deep Mutational Scanning Basics with Dr. Kenny Matreyek
- Learn about MaveDB - the public repository for datasets from Multiplexed Assays of Variant Effect (MAVEs)
- "Understanding the functional consequences of every mutation in Your Favourite Gene”
- "MaveDB: discovery and interpretation of high-throughput functional assay data”
Computational Resources
- Variant Effect Predictors https://www.varianteffect.org/vep/s
- MAVE Analysis Tools https://www.varianteffect.org/analysis-tools/
Monthly Seminar / Annual Meeting
Discussion Forums
Atlas of Variant Effects Alliance community slack channel (https://www.varianteffect.org/membership/)